This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and the choices between methods. It also provides a rational basis where several alternative methods are available.
Table of Contents
1. Introduction 2. Overview 3. Making Hybridomas 4. Monoclonal Antibodies: Production, Purification, Analysis, Quality Control, Storage, and Distribution 5. Using Monoclonal Antibodies: Cell and Tissue Markers 6. Using Monoclonal Antibodies: Soluble Antigens 7. Prospects, Problems, and Limitations in the Use of Monoclonal Antibodies