Practical Fluorescence Spectroscopy  book cover
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1st Edition

Practical Fluorescence Spectroscopy




ISBN 9781439821695
Published December 11, 2019 by CRC Press
772 Pages - 661 Color & 109 B/W Illustrations

 
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Book Description

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.

  • Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications
  • Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations
  • Covers steady-state phenomena, time-resolved phenomena, and advanced methods
  • Spans biophysical, biochemical, and biomedical applications
  • Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Table of Contents

Chapter 1. Theory of Light and Light Interaction with Matter 
Basics of Light
Light Polarization
Interaction of Light and Chromophores
Interactions Among Chromphores
Scattering
Summary

Chapter 2. Experimental Basics
Types of Cuvettes 
Types of Filters 
Measuring Transmittance/Absorbance
Factors Affecting the Precision of Absorption Measurement
Effect of Sample Scattering on Measured Transmission and Abosrbance
Measuring Emission 
Efficiency of Collecting Emission: Measuring Quantum Yield 
Wavelength Calibration
Instrucment Response
Lamp Profile
Peaks and Spectral Shapes 
Standard Deviation and Height 
Sums of Peaks
Noise–Signal-to-Noise Ratio
Sampling Rate
Bandwidth 
Artifacts and Errors 
Spectral Overlap
Peak Normalization
Residuals 
Interpolation

Chapter 3. Experiments
Introduction 
Experiments

Chapter 4. Fluorescence–Steady-State Phenomena
Introduction 
Experiments

Chapter 5. Steady-State Fluorescence: Applications
Introduction 
Experiments

Chapter 6. Steady-State Fluorescence Polarization: Anisotropy
Introduction 
How to Select and Check Polarizers 
Transition Moments
Polarized Absorption
Emission Polarization (Anisotropy)
Fundamental and Limiting Anistropies
Consequences of Linear Transition Moments
Determining the G-Factor for Spectrofluorometer
Experiments

Chapter 7. Fluorescence: Time-Resolved Phenomena
Introduction 
Experiments

Chapter 8. Advanced Experiments
Introduction 
Experiments

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Author(s)

Biography

Zygmunt (Karol) Gryczynski is a professor of molecular biology and immunology and the director of the Center for Commercialization of Fluorescence Technologies (CCFT) the University of North Texas Health Center.

Ignacy Gryczynski is a professor of cell biology and genetics and the director of the Microscopy Core Facility at the University of North Texas Health Center.